Polymeric-coated Fe-doped ceria/gold hybrid nanocomposite as an aptasensor for the catalytic enhanced colorimetric detection of 2,4-dinitrophenol

The Meisenheimer charge transfer reaction has, for many years, been the predominant mode of interaction to detect explosive compounds via naked eye. However, this method is majorly sensitive 2,4,6-trinitrotoluene and insensitive other compounds. In work, we report on development a novel aptamer-based amphiphilic polymer-coated Fe-doped ceria/gold (Au) hybrid nanocomposite (NC) as peroxidase mimetic nanozyme colorimetric detection. Polymeric-Fe-doped ceria NC was synthesized incorporated in-situ in hydrothermal synthetic pot citrate-Au nanoparticles form polymer-Fe-doped ceria/Au NC. A streptavidin (strep)-biotinylated DNA aptamer (B-Apt) binding complex immobilised surface modified used functional mimic assay catalyse oxidation 3,3′,5,5′-tetramethylbenzidine by H2O2. Screening several explosives showed that 2,4-dinitrophenol (DNP) bonded with higher affinity based selective greenish catalytic observed. activities nanozymes were tested our analysis generated enhanced activity DNP Several optimisation reactions including kinetic mechanism conducted limit detection 0.45 µg/mL (2.4 µM) obtained recognition. strep-B-Apt-polymer-Fe-doped biosensor successfully applied tap water river satisfactory recoveries range 97–117%.